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A group from the University of Zurich publish a new automated method for measuring the expression of genes in thousands of single human cells. The approach involves scaling up single-molecule fluorescence in situ hybridization, or smFISH, to determine where RNA transcripts are located in the cell. The scientists used brighter than normal probes, which allowed them to perform rapid and robust low-magnification imaging of many more cells, quantify low-level expression accurately, and also investigate very short RNA transcripts. Their software, meanwhile, automatically outlines cells and nuclei, counts dots to quantify expression, and documents where transcripts are located in the cell.